Oligo Library Synthesis

Oligonucleotide library synthesis with industry leading fidelity and representation agilent has a library solution to meet your needs.

Oligo library synthesis. Oligo pool synthesis many aspects of biological research such as genetic library construction and target enrichment capture in next generation sequencing ngs require a large amount of primers. Until now the demand for oligonucleotide based drugs has been fulfilled by conventional solid phase synthesis in an effective manner. Whereas enzymes synthesize dna and rna only in a 5 to 3 direction chemical. The advanced chemistries developed at agilent empower the creation of highly complex oligo pools and enable the printing of longer oligos.

This trimer oligonucleotide synthesis introduces a mixture of all 20 amino acid codons or subset at any location within the sequenced to be mutated. 1 coupling 3 deblock 2 oxidation depurination side reaction repeat n times n ho 1 n 1 n 0 p 2 ro 0 p 0 ro 0 p 0 ro 0 0 0 0 0 0 conventional processes. Proprietary dna synthesis equipment permits rapid high quality synthesis of nucleic acids. Conventional approaches to synthesizing hundreds of thousands of primers at a time are ineffective and time consuming.

Long oligo synthesis agilent has been a technological leader in the synthesis of longer oligos for the past decade. Dropsynth is a new gene synthesis method whereby large gene libraries are assembled from microarray derived oligo libraries within water in oil droplets. Previous gene synthesis methods require isolating individual gene assemblies in different reactions which becomes cost prohibitive for assembling thousands of genes and requires expensive automation equipment. Westphal chemical biology and therapeutics science program broad institute 415 main street cambridge massachusetts 02142 united states.

Research and development department otsuka medical devices co ltd kanda tsukasamachi chiyoda ku tokyo japan. Opools oligo pools are pooled high fidelity single stranded dna sequences that are compatible with a variety of applications. Water compatible cycloadditions of oligonucleotide conjugated strained allenes for dna encoded library synthesis matthias v. This platform is the same proprietary synthesis platform used to make idt ultramers.

As a result we have witnessed more than one hundred oligonucleotides currently in active clinical trials and eight food and drug administration fda approved drugs.